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Literature summary extracted from

  • Fang, H.M.; Zhao, L.; Lu, P.; Chen, S.J.; Bao, Z.X.; Qin, Y.F.; Zhu, Z.Y.; Zhao, J.M.; Mai, J.; Zhang, S.T.
    Modular design, expression and characterization of novel bifunctional mutants of fibrolase with combined platelet aggregation-inhibition and fibrinolytic activity (2011), Protein J., 30, 247-252.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.72 additional information the enzyme does not require plasminogen or any other bloodborne intermediate for activity Agkistrodon contortrix contortrix

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.72 chimeric Arg-Gly-Asp-fibrolase is expressed in Escherichia coli BL21(DE3) cells Agkistrodon contortrix contortrix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Zn2+ contains one zinc ion in the active center Agkistrodon contortrix contortrix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.72 23000
-
 x * 23000, SDS-PAGE Agkistrodon contortrix contortrix

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.72 Fibrinogen + H2O Agkistrodon contortrix contortrix the enzyme has a direct proteolytic activity against the A alpha-chain of fibrinogen at positions Lys413 and Leu414 ?
-
 ?
3.4.24.72 additional information Agkistrodon contortrix contortrix chimeric Arg-Gly-Asp-fibrolase efficiently inhibits platelet aggregation in a dose-dependent manner and a prolonged incubation time obviously increased the inhibitory effect, in contrast to fibrolase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Agkistrodon contortrix contortrix P28891
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 HiTrap Q-Sepharose column chromatography, gel filtration Agkistrodon contortrix contortrix

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.24.72 using 50 mM Tris-HCl, 0.5 M L-arginine, 1 M NaCl, 0.5 mM ZnCl2, 1 mM oxidized glutathione, 2 mM reduced glutathione, and 2 M urea at pH 7.0. After the protein is added, the pH is adjusted to 7.5, and the protein is left to refold at 4°C for approximately 24 h Agkistrodon contortrix contortrix

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.72 venom
-
 Agkistrodon contortrix contortrix
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 Fibrinogen + H2O the enzyme has a direct proteolytic activity against the A alpha-chain of fibrinogen at positions Lys413 and Leu414 Agkistrodon contortrix contortrix ?
-
 ?
3.4.24.72 additional information chimeric Arg-Gly-Asp-fibrolase efficiently inhibits platelet aggregation in a dose-dependent manner and a prolonged incubation time obviously increased the inhibitory effect, in contrast to fibrolase Agkistrodon contortrix contortrix ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.72 ? x * 23000, SDS-PAGE Agkistrodon contortrix contortrix